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Changes in protein synthesis during the initial stage of life of tobacco protoplasts
Authors:J Fleck  A Durr  M C Lett  L Hirth
Institution:(1) Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire, C.N.R.S., 15, Rue Descartes, F-67000 Strasbourg, France
Abstract:The biosynthesis of Fraction I protein in isolated protoplasts is compared with that in the plant. Radioactive precursors were incorporated into isolated protoplasts (ldquoin vitrordquo labeling) and into leaves, from which the protoplasts were isolated later (ldquoin siturdquo labeling). The biosynthesis of Fraction I protein stopped almost completely as soon as the protoplasts were incubated in the culture medium.
Keywords:Fraction I protein  Nicotiana  Protein synthesis  Protoplasts
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