Strategies to obtain stable transgenic plants from non-embryogenic lines: complementation of the nn
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mutation of the NORK gene in Medicago sativa MN1008 |
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Authors: | Ariana Perhald Gabriella Endre Zoltan Kevei Gyorgy B Kiss Attila Kereszt |
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Institution: | (1) Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari korut 62, 6726 Szeged, Hungary;(2) Present address: ARC Centre of Excellence for Integrative Legume Research, The University of Queensland, St. Lucia QLD, 4072 Queensland, Australia |
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Abstract: | Strategies to introduce genes into non-embryogenic plants for complementation of a mutation are described and tested on tetraploid alfalfa (Medicago sativa). Genes conditioning embryogenic potential, a mutant phenotype, and a gene to complement the mutation can be combined using several different crossing and selection steps. In the successful strategy used here, the M. sativa genotype MnNC-1008(NN) carrying the recessive non-nodulating mutant allele nn
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was crossed with the highly embryogenic alfalfa line Regen S and embryogenic hybrid individuals were identified from the F1 progeny. After transformation of these hybrids with the wild-type gene (NORK), an F2 generation segregating for the mutation and transgene were produced. Plants homozygous for the mutant allele and carrying the wild-type NORK transgene could form root nodules after inoculation with Sinorhizobium meliloti demonstrating successful complementation of the nn
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mutation. |
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Keywords: | Medicago Stable transformation Embryogenicity Nodulation Mutant Complementation |
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