| 高良姜素对人食管鳞癌KYSE-510细胞的抑制作用 |
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| 引用本文： | 王竹君,张 强,赵新淮. 高良姜素对人食管鳞癌KYSE-510细胞的抑制作用[J]. 中国生物化学与分子生物学报, 2009, 25(6): 563-569 |
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| 作者姓名： | 王竹君 张 强 赵新淮 |
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| 作者单位： | 乳品科学教育部重点实验室，东北农业大学，哈尔滨150030 |
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| 摘 要： | 探讨在体外高良姜素对人食管鳞癌KYSE-510细胞的抑制作用以及可能的作用机制.MTT结果表明，高良姜素对人食管鳞癌KYSE-510细胞具有很强的生长抑制作用.光学显微镜、激光共聚焦显微镜以及流式细胞仪的分析结果表明，高良姜素可诱导KYSE-510细胞分化.荧光定量RT-PCR和Western印迹分析结果表明，高良姜素诱导P21waf1、抑制细胞周期蛋白B1和细胞周期蛋白D1的表达，推测上述基因可能是高良姜素实现细胞分化诱导作用的靶基因.
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| 关 键 词： | 高良姜素 人食管鳞癌 分化 作用机制 |
| 收稿时间： | 2008-10-28 |
| 修稿时间： | 2008-12-29 |
Suppressive Effect of Galangin on Human Esophageal Squamous Cell Carcinoma Cell Line KYSE-510 |
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| WANG Zhu-Jun,ZHANG Qiang,ZHAO Xin-Huai. Suppressive Effect of Galangin on Human Esophageal Squamous Cell Carcinoma Cell Line KYSE-510[J]. Chinese Journal of Biochemistry and Molecular Biology, 2009, 25(6): 563-569 |
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| Authors: | WANG Zhu-Jun ZHANG Qiang ZHAO Xin-Huai |
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| Affiliation: | Key Laboratory of Dairy Science，Ministry of Education，Northeast Agricultural University，Harbin 150030，China |
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| Abstract: | Suppressive effect and the possible mechanism of galangin on human esophageal squamous cell carcinoma cell line KYSE-510 in vitro were studied.The result of MTT assay showed that galangin could greatly inhibit the growth and proliferation of KYSE-510 cells.Effect of galangin on induction of differentiation in KYSE-510 cells was determined by phase contrast microscopy，confocal laser scanning microscopy and flow cytometry.The results of real-time RT PCR and Western blot analysis showed that galangin might induce expression of P21waf1 and inhibit the expressions of cyclin B1 and cyclin D1.It was speculated that P21waf1，cyclin B1 and cyclin D1 might be target genes of galangin in inducing cell differentiation. |
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| Keywords: | galangin human esophageal squamous carcinoma differentiation mechanism |
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