Assessment of genetic diversity of pigeonpea cultivars using RAPD analysis |
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| Authors: | P Ray Choudhury I P Singh B George A K Verma N P Singh |
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| Institution: | (1) Biotechnology Unit, Indian Institute of Pulses Research, Kanpur, 208024, India;(2) Present address: Directorate of Seed Research, Mau, 275101 Uttar Pradesh, India |
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| Abstract: | In our present study assessment of genetic diversity and identification of pigeonpea cultivars has been done by employing
76 random amplified polymorphic DNA (RAPD) primers. Out of 796 amplified products, 587 showed polymorphism (73.7 %) and an
average of 10.47 bands were amplified per primer. Cluster analysis based on Jaccard’s similarity coefficient using UPGMA grouped
all the cultivars into three clusters. The cluster I consists of 7 cultivars, cluster II of 11 cultivars in 4 sub-clusters
and cluster III 4 cultivars. Two cultivars were not included in any cluster. The clustering was strongly supported by high
bootstrap values. Furthermore, high values of the average heterozygosity (Hav) and marker index (MI) also indicated the efficiency of RAPD as a marker system. |
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| Keywords: | Additional" target="_blank">Additional DNA fingerprinting molecular marker DNA amplification Cajanus cajan |
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