Addition of nutrients induce a fast loss of flocculation in starved cells of Saccharomyces cerevisiae

Incubation of flocculent cells of a brewing strain of Saccharomyces cerevisiae of the NewFlo phenotype for 8–12 h, in the absence of either carbon or nitrogen source, did not induce a loss of flocculation, although an increase (about two times) in the number of cells occurred in nitrogen starved cells. The addition of glucose or ammonium sulphate to carbon or nitrogen starved cells, respectively, triggered a rapid loss of flocculation.