Gene transfer by jet injection into differentiated tissues of living animals and in organ culture |
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Authors: | Priscilla A Furth David Kerr Robert Wall |
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Institution: | 1. Division of Infectious Diseases, Department of Medicine, University of Maryland School of Medicine and the Baltimore Veterans Administration Medical Center, 10 North Greene Street, 21201, Baltimore, MD 2. AltraBio Inc., Arden Hills, MN 3. Gene Evaluation and Mapping Laboratory, USDA-ARS, Beltsville, MD
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Abstract: | Jet injection can be used to introduce genes into the cells of differentiated tissues of living animals and organ cultures. When a solution of plasmid DNA is jet injected into a selected tissue or organ, cells lying in or near the path of the jet injection are transfected with the DNA and the introduced gene(s) are expressed. Since there is minimal morbidity from each jet injection, multiple injections can be performed at the same or nearby sites. Both mRNA and protein expression from transfected genes can be quantitated using standard methods. In addition, the technique is an efficient means of DNA immunization. Methodology for using jet injection to transfer plasmid DNA into the cells of skin, fat, mammary gland, and muscle are described. |
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