Induction,protein composition and DNA ploidy level of embryogenic calli of silver fir and its hybrids |
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| Authors: | A Gajdošová B Vooková A Kormuťák G LIbiaková J Doležel |
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| Institution: | 1. Institute of Plant Genetics, Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, 950 07, Nitra, Slovakia
2. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Sokolovská 6, 77200, Olomouc, Czech Republic
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| Abstract: | Somatic embryogenesis was initiated from immature zygotic embryos of intraspecific cross ofAbies alba and interspecific combination ofAbies alba x Abies nordmanniana. The most responsive explants for induction of embryogenic calli were zygotic embryos in precotyledonary stage of development. Biochemically the compared embryogenic lines were uniform irrespective of their morphology or embryogenic potential. The flow cytometric analysis of single embryogenic lines suggests that no changes in ploidy levels occur during induction and culture of embryogenic callus inAbies alba what confirms the convenience of this system for propagation of valuable seed material. |
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