Ribulose bisphosphate carboxylase activity in halophilic Archaebacteria |
| |
Authors: | Wijaya Altekar Rema Rajagopalan |
| |
Affiliation: | (1) Biochemistry Division, Bhabha Atomic Research Centre, 400 085 Bombay, India |
| |
Abstract: | Among the several strains of halobacteria grown heterotrophically, ribulose bisphosphate carboxylase activity was detected in those which accumulate poly (-hydroxybutyrate), viz. Haloferax mediterranei, Haloferax volcanii and Halobacterium marismortui. In H. mediterranei, the activity was present in cell extracts prepared after growth on a variety of carbohydrates. The ribulose bisphosphate carboxylase activity in H. mediterranei was inhibited by carboxyarabinitol bisphosphate, and the enzyme cross-reacted with antibodies raised against the spinach enzyme. CO2 fixation by cell extract was stimulated by the addition of ATP and NADH. Preliminary data suggested that hydrogen could be a possible reductant.Abbreviations RuBP ribulose bisphosphate - Ru5P ribulose 5-phosphate - R5P ribose 5-phosphate - CABP carboxyarabinitol bisphosphate - PHB poly (-hydroxybutyrate) - DTT dithiothreitol |
| |
Keywords: | Halophilic archaebacteria Haloferax mediterranei Ribulose bisphosphate carboxylase activity |
本文献已被 SpringerLink 等数据库收录! |
|