Mapping of additional restriction enzyme cleavage sites on bacteriophage MB78 genome |
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| Authors: | S S Murty B Pandey Maharani Chakravorty |
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| Institution: | (1) Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, 221 005 Varanasi, India |
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| Abstract: | A physical map of bacteriophage MB78 DNA indicating the cleavage sites for the enzymeBglII,ClaI,EcoRI,PvuII,SalI andSmaI comprising of a total of 34 cleavage sites have been constructed earlier. The cleavage sites for a few more restriction
endonucleases likeApaI,AvaI,BglI,HindIII,KpnI andXhoI have now been mapped. A total of 72 cleavage sites on MB78 DNA are known by now. Relative positions ofEcoRI I and J fragments which could not be decided earlier has now been determined. |
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| Keywords: | Bacteriophage MB78 physical map submolar fragment |
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