侵染落葵的黄瓜花叶病毒分离物CP基因序列分析

通过间接酶联免疫法(ID-ELISA)检测到染病落葵病样中存在黄瓜花叶病毒（Cucumber Mosaic Virus，CMV）。从病叶中提取总RNA，用RT-PCR方法扩增得到657bp的CMV CP基因片断，将扩增产物与T载体连接并进行测序。用DNA MAN将得到的CP基因序列与GenBank收录的黄瓜花叶病毒两亚组部分株系或分离物的CP基因序列进行比较，结果表明该CP基因与CMV亚组Ⅰ、亚组Ⅱ之间的核苷酸序列同源性分别为91.17~95.43%和75.30~75.76%，推导氨基酸序列同源性分别为95.41~97.71%和81.28~81.74%，表明CMV-Ba与亚组Ⅰ同源关系密切。

Sequence Analysis of Coat Protein Gene of Cucumber Mosaic Virus Isolate Infecting Basella rubra L.

Cucumber mosaic virus was detected from infected Basella rubra L. with the indirect enzyme-linked immunosorbent assay. Total RNA was extracted from infected leaves and the cDNAs of coat protein gene of CMV-Ba were obtained by RT-PCR. The amplified cDNA fragments were then cloned into pMD 18-T vector and sequenced，the result showed that the CP gene was 657 nucleotides in length. This sequence was aligned with the obtained CP gene and some CMV strains or isolates of subgroup Ⅰ and subgroup Ⅱ in GenBank using DNA MAN software. The results showed that CMV-Ba shared 90.9% -93.8% and 76.1%-76.9% identity with the known CP genes of subgroup Ⅰ and Ⅱ respectively in nucleotide level，on the other hand，amino acids deduced from CMV-Ba CP gene shared 92.7% -97.7% and 72.4%-78.1% identity with the known CP protein of subgroup Ⅰ and Ⅱ，respectively. This suggested that CMV-Ba CP gene belongs to CMV subgroupⅠ.