Interleukin-2 induces the activities of DNA topoisomerase I and DNA topoisomerase II in HuT 78 cells |
| |
Authors: | Minh-Hieu Pham |
| |
Affiliation: | a Department of Biology, UPO #311, University of the Incarnate Word, 4301 Broadway, San Antonio, TX 78209-6318, USA b Biology Department, Rutgers University, 311 North Fifth Street, Camden, NJ 08102-1405, USA |
| |
Abstract: | The induction by interleukin-2 of DNA topoisomerase I and DNA topoisomerase II activities in the human T cell line HuT 78 was investigated. HuT 78 cells were treated with 1000 U of interleukin-2/ml, and extracts of the HuT 78 nuclei were prepared over a 24 h period. The extracts were assayed quantitatively for the activities of DNA topoisomerase I and DNA topoisomerase II. Three concomitant, transient increases of 3- to 11-fold in the specific activities of both DNA topoisomerase I and DNA topoisomerase II were observed following treatment with IL-2 at 0.5, 4, and 10 h after treatment with interleukin-2. The specific activities of both enzymes returned to base-line values after each of these transient increases. These results reveal that the activities of DNA topoisomerase I and DNA topoisomerase II are highly regulated in HuT 78 cells upon treatment with IL-2. |
| |
Keywords: | DNA topoisomerase I DNA topoisomerase II Interleukin-2 |
本文献已被 ScienceDirect 等数据库收录! |
|