Invertase and phosphatase of yeast in a phosphate-limited continuous culture |
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Authors: | Kiyoshi Toda Isamu Yabe Toshihiko Yamagata |
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Affiliation: | (1) Institute of Applied Microbiology, University of Tokyo, Yayoi 1-1-1, Bunkyoku, Tokyo |
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Abstract: | Summary Deficiency of inorganic phosphate caused the hyper production of invertase and the derepression of acid phosphatase in a continuous culture ofSaccharomyces carlsbergensis. The specific invertase activity was 40,000 enzyme units per g dry cell weight at a dilution rate lower than 0.05 h–1 with a synthetic glucose medium of which the molecular ratio of KH2PO4 to glucose was less than 0.006. This activity is eight fold higher than in a batch growth and 1.5 fold as much as the highest enzyme activity observed so far in a glucose-limited continuous culture.For the hyper production of invertase, it is necessary to culture the yeast continuously by keeping the Nyholm's conservative inorganic phosphate concentration at less than 0.2 m mole per g dry weight cell. The derepression of acid phosphatase brought about by phosphate deficiency, was similar in both batch and continuous cultures.Nomenclature D dilution rate of continuous culture (h–1) - Ei invertase concentration in culture (enzyme unit l–1) - Ep acid phosphatase concentration in culture (enzyme unit l–1) - P inorganic phosphate concentration in culture (mM) - S glucose concentration in culture (mM) - X cell concentration in culture (g dry weight cell l–1)Greek Letter specific rate of growth (h–1)Suffix f feed - 0 initial value |
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