Amplification and characterization of bull semen infected naturally with foot-and-mouth disease virus type Asia1 by RT-PCR |
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Authors: | Jun-jun Shao Hui-yun Chang Tong Lin Guo-zheng Cong Jun-zheng Du Jian-hong Guo Hui-fang Bao You-jun Shang Ya-min Yang Xiang-tao Liu Zai-xin Liu Ji-xing Liu |
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Institution: | (1) Key Laboratory of Animal Virology of Minister of Agriculture, State key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China |
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Abstract: | To investigate the security of semen biologically, 15 bull semen samples were collected (of which 5 exhibited clinical signs
of Foot-and-mouth disease) and identified by RT-PCR and virus isolation. The results indicated that the semen of the infected
bulls were contaminated by Foot-and-mouth disease virus (FMDV), but FMDV was not detected in semen samples from those bulls
not showing clinical signs of Foot-and-mouth disease (FMD). This is the first report of the presence of FMDV in bull semen
due to natural infection in China. The analysis of the partial sequence of the VP1 gene showed that the virus strain isolated
from semen has 97.9% identity with the virus isolated from vesicular liquid of infected bulls showing typical signs of FMD
and belonged to the same gene sub-group.
Foundation items: State Science and Technology Support Program (2006DAD06A03) and Hi-tech Research and Development Program
of China 863 (2006AA10A204). |
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Keywords: | Bull Foot-and-mouth disease (FMD) Foot-and-mouth disease virus (FMDV) RT-PCR Semen |
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