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A physical map of the ribosomal DNA repeat unit of Aspergillus nidulans |
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| Authors: | Robin A Lockington Graham G Taylor Michael Winther Claudio Scazzocchio RWayne Davies |
| Institution: | 1. University of Essex, Department of Biology, Wivenhoe Park, Colchester, Essex, C04 3SQ, P.O. Box 88, Manchester M60 1QD U.K.;2. University of Manchester Institute of Science and Technology, Department of Biochemistry and Molecular Biology P.O. Box 88, Manchester M60 IQD U.K. |
| Abstract: | The ribosomal DNA repeat unit of Aspergillus nidulans has been cloned in pBR322 and a restriction map constructed. The genes coding for the 17S, 5.8S and 25S rRNAs are found in blocks separated by a 1.7 kb spacer region, with the 5.8S RNA gene lying between the genes for the two larger RNAs. The total length of the repeat unit is 7.7 kb. The 5S rRNA is not present in the repeat unit. |
| Keywords: | pBR322 gene library recombinant DNA bp base pairs kb kilobase pairs rDNA DNA coding for ribosomal RNA rRNA ribosomal RNA |
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