Phosphorescent analysis of lipid peroxidation products in liposomes] |
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| Authors: | V M Mazhul' D G Shcherbin |
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| Affiliation: | Institute of Photobiology, Belarussian Academy of Sciences, Minsk, Belarus. |
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| Abstract: | It was found that lipid peroxidation products incorporated into liposomes prepared from oxidized preparations of bovine heart phosphatidylcholine and the total lipid fraction of human erythrocyte membranes are able to phosphoresce at room temperature was studied. The temperature dependences of kinetic and spectral parameters of phosphorescence were measured. It is shown that mechanism of phosphorescence quenching of lipid chromophores has a dynamic nature. It is proposed to use endogenic molecules of the lipid peroxidation products capable of phosphorescence as intrinsic phosphorescence probes for studying the slow molecular dynamics of lipids in artificial and biological membranes in a millisecond range. |
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