Terpenoid Metabolism in Plastids : Localization of alpha-Tocopherol Synthesis in Capsicum Chromoplasts |
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Authors: | Camara B Bardat F Seye A D'Harlingue A Monéger R |
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Institution: | Laboratoire de Régulations Métaboliques et Différenciation des Plastes, Tour 53, 2ème Etage, Université Pierre et Marie Curie (Paris 6) 4, Place Jussieu, 75230 Paris Cédex 05. |
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Abstract: | The synthesis of α-tocopherol from 2,3-dimethylphytylquinol and S-adenosyl-l-methionine was achieved using Capsicum annuum fruit chromoplasts. The enzymes involved in the cyclization (2,3-dimethyl-phytylquinol cyclase) and methylation (S-adenosyl methionine:γ-tocopherol methyl-transferase) are both localized in the chromoplast membrane fraction (envelopes and/or a-chlorophyll lamellae), in contrast to the stroma fraction. |
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