Structural identification of sulfated tyrosine in human urine |
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Authors: | Ranasinghe J G Sakakibara Y Harada M Nishiyama K Liu M C Suiko M |
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Institution: | Department of Biological Resource Sciences, Miyazaki University, Japan. |
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Abstract: | A reliable HPLC method was used for the identification of positional isomerism and stereoisomerism of sulfated tyrosine residues in human urine. Upon separation of human urine by ion-pair HPLC on a reverse-phase column, p-tyrosine-O-sulfate (p-TyrS) was identified. Differentiation of the L and D forms was done by using a column with a chiral stationary phase. It was concluded that L-p-tyrosine (L-p-Tyr) which is the predominant tyrosine isomer in the human body, was sulfated and excreted in human urine as a normal constituent. The sulfated forms of D-p-Tyr and m-Tyr could not be detected under these analytical conditions. |
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