The demonstration of B cells in pancreatic islets with Orcein |
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Authors: | F. Callea and V. J. Desmet |
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Affiliation: | (1) Laboratorium voor Histochemie en Cytochemie, Department Medische Navorsing, Katholieke Universiteit Leuven, 3000 Leuven, Belgium |
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Abstract: | Summary Orcein stains granules in pancreatic islet cells selectively. The localization of Orcein-positive cells within islets differs from that of Grimelius-stained cells, but corresponds to the B cell type differentiated by Aldehyde Fuchsin.Usually there appear to be fewer Orcein-positive cells than Aldehyde Fuchsin-positive ones. This indicates either that Aldehyde Fuchsin is a more sensitive stain for B cells or that Orcein is a more selective stain for a B cell type subpopulation. The rationale of the Orcein reaction in B cells seems to depend on the oxidation of disulphide bonds present in insulin and its precursors rich in cystine. |
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