Molecular detection and direct enumeration of methanogenic Archaea and methanotrophic Bacteria in domestic solid waste landfill soils |
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| Authors: | Ann-Cheng Chen Kumiko Ueda Yuji Sekiguchi Akiyoshi Ohashi Hideki Harada |
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| Institution: | (1) Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan;(2) Department of Environmental Engineering and Science, Chia-Nan University of Pharmacy and Science, 60 Erh-Jen Road, Sec. 1, Pao-An, Jen-Te, Tainan Hsien, 717, Taiwan;(3) Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8566, Japan |
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| Abstract: | Methane oxidizing and producing activities of cover soil (10, 30 cm depth) and burial waste (1, 3 m depth) were evaluated: top cover soil (10 cm) had the highest methane oxidizing activity, while 1 m depth buried waste showed the highest methane producing potential. All the sequences of the 1 m sample were found to be closely related to 16S rDNAs of mainly hydrogenotrophic methanogens known, such as genera Methanosarcina, Methanoculleus, and Methanobacterium. We developed a modified fluorescence in situ hybridization (FISH) direct counting method for landfill samples, resulting in the detection of approx. 1% of total cells as archaeal cells (presumably methanogens). However, probe-positive cells could not be found with probes for methanotrophs by the methods. |
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| Keywords: | archaea enumeration fluorescence in situ hybridization landfill methanotrophs |
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