Physical incorporation of a single-stranded oligodeoxynucleotide during targeted repair of a human chromosomal locus |
| |
Authors: | Radecke Sarah Radecke Frank Peter Ingrid Schwarz Klaus |
| |
Affiliation: | Institut für Klinische Transfusionsmedizin und Immungenetik Ulm. Abteilung Transfusionsmedizin, Universit?tsklinikum Ulm, D-89081 Ulm, Germany. |
| |
Abstract: | BACKGROUND: Targeted gene repair is an attractive method to correct point-mutated genes at their natural chromosomal sites, but it is still rather inefficient. As revealed by earlier studies, successful gene correction requires a productive interaction of the repair molecule with the target locus. The work here set out to investigate whether DNA repair, e.g., mismatch repair, or a direct incorporation of the correction molecule follows as the step upon the initial interaction. METHODS: Single-stranded 21mer oligodeoxynucleotides (ODNs) of sense orientation were directed towards point-mutated enhanced green fluorescence protein transgene loci in HEK-293-derived cell clones. First gene repair assays compared ODNs carrying the canonical termini 5'-phosphate and 3'-OH with their respective variants harbouring non-canonical termini (5'-OH, 3'-H). Second, a protocol was established to allow efficient recovery of integrated short biotin-labelled ODNs from the genomes of gene-corrected cells using streptavidin-coated beads in order to test directly whether transfected ODNs become bona fide parts of the target locus DNA. RESULTS: Oligodeoxynucleotides with canonical termini were about 34-fold more efficient than their counterparts carrying non-canonical termini in a phosphorothioate-modified backbone. Furthermore, biotinylated fragments were successfully recovered from genomic DNAs of gene-corrected cells. CONCLUSIONS: The experiment showed that ODNs are incorporated into a mammalian genome. This unravels one early repair step and also sets an unexpected example of genome dynamics possibly relevant to other ODN-based cell techniques. |
| |
Keywords: | dideoxy terminus Okazaki fragment oligonucleotide incorporation oligonucleotide‐mediated gene correction |
本文献已被 PubMed 等数据库收录! |
|