C-21 steroidal glycosides from the roots of Cynanchum chekiangense and their immunosuppressive activities |
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Authors: | Li Xiaoyu Sun Hongxiang Ye Yiping Chen Fengyang Pan Yuanjiang |
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Affiliation: | Department of Chemistry, Zhejiang University, Hangzhou 310027, PR China. |
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Abstract: | Two new C21 steroidal glycosides, chekiangensosides A and B, were isolated from the roots of Cynanchum chekiangense, together with two known compounds. On the basis of chemical evidence and extensive spectroscopic methods, including one-dimensional and two-dimensional NMR, the structures of two new compounds were identified as cynajapogenin A, 3-O-beta-D-glucopyranosyl-(1-->4)-beta-D-cymaropyranosyl-(1-->4)-alpha-L-cymaropyranosyl-(1-->4)-beta-D-cymaropyranoside, and glaucogenin A, 3-O-beta-D-glucopyranosyl-(1-->4)-beta-D-cymaropyranosyl-(1-->4)-alpha-L-cymaropyranosyl-(1-->4)-beta-D-cymaropyranoside, respectively. The two known steroidal glycosides, and were revised. These isolated compounds were tested for their immunological activities in vitro against concanavalin A (Con A)- and lipopolysaccharide (LPS)-induced proliferation of mice splenocytes. Compounds showed immunosuppressive activities in vitro in a dose-dependent manner. |
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Keywords: | Con A, concanavalin A cym, cymaropyranosyl dig, digitoxopyranosyl glc, glucopyranosyl LPS, lipopolysaccharide MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide OVA, ovalbumin |
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