A simple, sensitive method for measuring 3,4-dihydroxyphenylacetic acid and homovanillic acid in rat brain tissue using high-performance liquid chromatography with electrochemical detection. |
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| Authors: | F Hefti |
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| Affiliation: | Laboratory of Neuroendocrine Regulation Department of Nutrition and Food Science Massachusetts Institute of Technology Cambridge, Massachusetts 02139, USA |
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| Abstract: | A sensitive, specific, and very simple method, using high-performance liquid chromatography combined with electrochemical detection, measured 3,4-dihydroxyphenylacetic acid and homovanillic acid in small areas of rat brain. The dopamine metabolites were extracted with diethyl ether and a known amount of vanillic acid as internal standard, and were separated on a micro-particulate reverse-phase column using a sodium acetate buffer (pH 5.0) as mobile phase. |
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