Inorganic phosphate nanorods are a novel fluorescent label in cell biology |
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Authors: | Chitta Ranjan Patra Resham Bhattacharya Sujata Patra Sujit Basu Priyabrata Mukherjee Debabrata Mukhopadhyay |
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Institution: | (1) Department of Biochemistry and Molecular Biology, Mayo Clinic Cancer Center, Mayo Clinic, Rochester, Minnesota, USA |
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Abstract: | We report the first use of inorganic fluorescent lanthanide (europium and terbium) ortho phosphate LnPO4·H2O, Ln = Eu and Tb] nanorods as a novel fluorescent label in cell biology. These nanorods, synthesized by the microwave technique,
retain their fluorescent properties after internalization into human umbilical vein endothelial cells (HUVEC), 786-O cells,
or renal carcinoma cells (RCC). The cellular internalization of these nanorods and their fluorescence properties were characterized
by fluorescence spectroscopy (FS), differential interference contrast (DIC) microscopy, confocal microscopy, and transmission
electron microscopy (TEM). At concentrations up to 50 μg/ml, the use of 3H]-thymidine incorporation assays, apoptosis assays (TUNEL), and trypan blue exclusion illustrated the non-toxic nature of
these nanorods, a major advantage over traditional organic dyes |
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