Allyl Chloride-Induced Time Dependent Changes of Lipid
Peroxidation in Rat Nerve Tissue |
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Authors: | Qing-Shan?Wang Email author" target="_blank">Ke-Qin ?XieEmail author Cui-li?Zhang Ying-Jian?Zhu Li-Ping?Zhang Xin?Guo Su-fang?Yu |
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Institution: | (1) Institute of Toxicology, Shandong University, 44 West Wenhua Road, Jinan, 250012, P.R. China;(2) Institute of Toxicology, Shandong University, 44 West Wenhua Road, Jinan, 250012, P.R. China |
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Abstract: | To accurately know the time-dependent changes of the lipid peroxidation and antioxidative status for elucidating the mechanism
of neuropathy induced by allyl chloride (AC), the malondialdehyde (MDA), anti-reactive oxygen species (anti-ROS), glutathione
(GSH), catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) were investigated in cerebrum, spinal cord
and sciatic nerve of rats after 0, 3, 6, 9, 12 weeks of␣AC administration. AC was administrated to Wistar rats by gavage at
a single dosage of 200 mg/kg/per dose (three times per week). Rats were sacrificed after 0, 3, 6, 9, 12 weeks of treatment,
and cerebrum, spinal cord, sciatic nerves were dissected, homogenized and used for the determination of lipid peroxidation
and antioxidative status. The results showed that MDA in cerebrum (112.4%) and sciatic nerve (113.1%) significantly increased
(P < 0.05) on third week of AC treatment and at gait score of 2, and further changes of MDA were observed after 6, 9, 12 weeks
and at gait score of 3, 4. While a decrease (P < 0.05) in the activities of GSH, CAT, GPx and SOD after 6, 9, 12 weeks intoxication and at gait score of 2, 3, 4 were observed
in cerebrum, spinal cord and sciatic nerve. Anti-ROS activities also decreased in all three nerve tissues after 3, 6, 9, 12 weeks
intoxication and at gait score of 2, 3, 4. Thus, AC intoxication was associated with elevation of lipid peroxidation and reduction
of antioxidative status, and the time-dependent changes of these indexes in Wistar rats nerve tissues occurred. Sciatic nerve
was the main target tissue and MDA was most sensitive among all indexes. The changes of lipid peroxidation and antioxidative
status might be related to the degradation of nerve fiber and served as one of mechanisms of toxic neuropathy induced by AC. |
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Keywords: | Allyl chloride lipid peroxidation MDA SOD GSH |
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