生物质谱在细胞信号转导研究中的应用

近几年快速发展起来的生物质谱技术 ,依靠 (酶解后肽段 )精确质量数测定和随机肽序列标签分析 ,实现了对蛋白质高通量的鉴定 ,并被成功地用于蛋白质相互作用和蛋白质磷酸化等翻译后修饰研究。与传统的研究手段相比 ,上述技术能够在一次实验中对多信号通路中所有磷酸化的蛋白质分子及其磷酸化位点进行鉴定 ,已成为蛋白质组学最新发展中令人关注的一个热点。简要综述质谱技术应用于上述工作中的 3种策略

Application of Bio mass Spectrometry in Cellular Signal Transduction

The quickly developing techniques of biological mass spectrometry (bio MS) in recent years realized the high throughput identification of proteins by determining the accurate mass values of trypsin digested peptides and the randomly selected peptide sequence tags, and have been successfully used in the studies of protein interactions and post translational modification such as the phosphorylation. Compared to the conventional approaches, the above techniques can identify all the phosphorylated proteins (including their phosphorylated amino acid sites) involved in a multi signal pathway in a single experiment, and they have been developed into a hot spot of proteomics. The three strategies for the application of bio MS in the above fields are briefly reviewed.