| 一种提取蜘蛛基因组DNA的有效方法 |
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| 引用本文： | 陈国亮,邵爱云,孟清.一种提取蜘蛛基因组DNA的有效方法[J].动物学杂志,2007,42(6):64-68. |
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| 作者姓名： | 陈国亮 邵爱云 孟清 |
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| 作者单位： | 东华大学生物科学与技术研究所,上海,201620;东华大学生物科学与技术研究所,上海,201620;东华大学生物科学与技术研究所,上海,201620 |
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| 基金项目： | 国家高技术研究发展计划(863计划)资助项目(No2006AA03Z0451) |
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| 摘 要： | 介绍了用尿素法提取蜘蛛基因组DNA。通过与其他DNA提取方法相比较,证明尿素法具有可在室温条件下进行、DNA得率高、完整性好、简单快速等优点。以提取的DNA为模板进行PCR扩增,获得预期大小的、高重复、高GC含量的编码蜘蛛牵引丝蛋白基因的DNA片段。
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| 关 键 词： | 蜘蛛 基因组DNA DNA提取 PCR扩增 |
| 文章编号： | 0250-3263(2007)06-64-05 |
| 收稿时间： | 7/5/2007 12:00:00 AM |
| 修稿时间： | 2007年7月5日 |
A Efficient Method for Extraction of Spiders' Genomic DNA |
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| CHEN Guo-Liang,SHAO Ai-Yun and MENG Qing.A Efficient Method for Extraction of Spiders' Genomic DNA[J].Chinese Journal of Zoology,2007,42(6):64-68. |
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| Authors: | CHEN Guo-Liang SHAO Ai-Yun and MENG Qing |
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| Institution: | Institute of Biological Sciences and Biotechnology,Donghua University,Shanghai 201620,China;Institute of Biological Sciences and Biotechnology,Donghua University,Shanghai 201620,China;Institute of Biological Sciences and Biotechnology,Donghua University,Shanghai 201620,China |
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| Abstract: | The present paper deals with the urea lyses method for isolating spiders' genomic DNAs.Compared to other two known methods,this method produced spider genomic DNAs with higher yields and higher integrity.It is also simpler and less time-consuming,involving only room temperature manipulations.High quality genomic DNAs were isolated from spider by this method.Certain spider dragline gene fragments,which contain highly repetitive sequences of high GC contents,were successfully PCR-amplified. |
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| Keywords: | Spider Genomic DNA DNA extraction PCR amplification |
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