细胞周期调控因子在非小细胞肺癌作用的研究

目前研究认为P16、视网膜母细胞瘤基因（Rb）、细胞周期蛋白（CylinD1）及细胞周期蛋白依赖性激酶（CDK4）几种细胞周期调控因子相互作用构成一条重要的细胞周期调节通路，为了解P16、PRb、CyclinD1种调控因子在非小细胞肺癌（NSCLC）中的作用，本实验采用免疫组织化学方法对38例原发性NSCLC中上述三种因子的表达进行了研究。结果，其中54％的肺癌组织出现CyclinD1的过度表达，P16、Rb的阳性表达率分别为47．4％和76．3％。我们发现，在50％Rb阳性病例中，P16蛋白不表达或表达水平很低，在21％PRb阴性病例中，P16蛋白具有较高的表达水平，本研究结果提示：1．PRb与P16蛋白在NSCLC中的表达呈负相关，P16的表达可能受PRb负调控，2．PRb的失活与CyclinD1过度表达共同存在于NSCLC中；3．NSCLC的发生涉及P16－Rb－CyclinD1／CDK4调节通路多个调控因子的异常。

THE EFFECT OF CELL CYCLE REGULATORS IN PRIMARY NON-SMALL CELL LUNG CANCER

P16. PRb .Cyclin D1 and CDK4 intract with each other forming one important regulatory pathway of cell cycle. We examined 38 cases of primary non-small lung cancer (NSCLC) bymeans of immunohistochemistry to study the function of P16. PRb and Cyclin D1 in the oncogenesis of NSCLC. Overexpression of Cyclin D1 was seen in 54% of the cases. The rate of positive expression of P16 and PRb was 47. 4% and 76. 3% respectively. 50% of PRb positive cases had no or low P16 expression, while 21 % of PRb negative cases showed high level of P16. These results suggest: 1. PRb is inversely correlated with P16 in NSCLC and P16 may be negatively regulated by functional PRb; 2. the loss of PRb coexist with the overexpression of cyclin D1 in NSCLC; 3. the abnormality of more regulators in this regulatory pathway contributes to the oncogenesis of NSCLC.