Interaction of sea cucumber saponins with multilamellar liposomes |
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Authors: | Byung Sul Yu In-Ho Jo |
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Affiliation: | Department of Physical Pharmacy, College of Pharmacy, Seoul National University, San 56-1, Shinrim-Dong, Kwanak-Ku, Seoul Korea |
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Abstract: | The effect of three sea cucumber saponins, echinoside A, bivittoside D and holothurin A, on multilamellar liposomes was investigated. An ideal osmotic behavior of liposomes was described as a linear relationship between the reciprocal power of absorbance at 450 nm and the osmotic gradient across the membrane. Sea cucumber saponins at concentrations below critical micelle concentration (CMC) disturbed this linear relationship in liposomes composed of egg phosphatidylcholine, phosphatidic acid and cholesterol. Cholesterol-free liposomes were not susceptible to these saponins. Results of optical measurements were consistent with those of transmission electron microscopy, which showed saponin-induced changes in liposomal structure. The lytic activity of sea cucumber saponins on liposomes depended on their chemical structure.These results suggest that sea cucumber saponins as monomers can interact with liposomes and that cholesterol serve as a principal binding site for the sea cucumber saponins. |
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Keywords: | Multilamellar liposomes - Osmotic behavior - Sea cucumber saponins ANS, 8-anilino-naphthalene sulfonate Ch, cholesterol CMC, critical micelle concentration PA, phosphatidic acid PC, egg phosphatidylcholine |
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