A new method to perform nonradioactive tissue printing using digoxigenin-labeled riboprobes |
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| Authors: | Christine Tiré Marc Van Montagu Gilbert Engler |
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| Affiliation: | (1) Laboratorium voor Genetica, France;(2) Laboratoire associé de l'Institut National de la Recherche Agronomique, France;(3) Universiteit Gent, B-9000 Gent, (Belgium) |
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| Abstract: | We describe a modified protocol to perform a tissue print northern using digoxigenin-labeled riboprobes. The result of the hybridization is directly visualized on the membrane by an alkaline phosphatase detection system. |
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| Keywords: | digoxigenin extensin tissue print |
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