Deletion/insertion polymorphism of the prion protein gene (<Emphasis Type="Italic">PRNP</Emphasis>) in Polish Red cattle,Polish White-backed cattle and European bison (<Emphasis Type="Italic">Bison bonasus</Emphasis> L., 1758) |
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Authors: | U Czarnik G Grzybowski T Zabolewicz J Strychalski S Kaminski |
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Institution: | (1) Department of Animal Genetics, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-719, Poland;(2) Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, Wolka Kosowska, 05-552, Poland |
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Abstract: | The aim of the present study was to identify deletion/insertion polymorphism of the bovine prion protein (PRNP) gene within the promoter sequence (23 bp indel), intron 1 (12 bp indel) and the 3′ end untranslated region (14 bp indel).
The experiment was performed on three groups of animals protected under a genetic resources conservation program: 139 Polish
Red (PR) cows, 79 Polish White-backed cows and 50 European bison (Bison bonasus L., 1758). White-backed cattle were characterized by a higher frequency of ins/del heterozygotes and a relatively lower frequency
of ins/ins homozygotes within the promoter sequence region (23 bp indel), compared to Polish Red cattle. At the polymorphic
locus of intron 1 (12 bp indel) the genetic structure of both cattle populations was similar. Monomorphism, expressed by the
occurrence of one genotype variant in each of the analyzed sequence regions, was observed in European bison. Five haplotypes
were found in Polish White-backed cows, four haplotypes in Polish Red cows and only one in analyzed group of bison. Differences
between the observed and expected number of PRNP haplotypes were recorded in Polish Red cattle.
The article is published in the original. |
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