Molecular cloning of Na(+)-ATPase cDNA from a marine alga, Heterosigma akashiwo |
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Authors: | Shono M Wada M Hara Y Fujii T |
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Institution: | Japanese International Research Center for Agricultural Sciences-Okinawa Subtropical Station, Ishigaki, Japan. mshono@jircas-os.affrc.go.jp |
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Abstract: | We cloned novel Na(+)-ATPase (HANA) cDNA from marine alga Heterosigma akashiwo. The full-length HANA cDNA was 4467 bp long and coded for a 1330 amino acid protein with a molecular weight of 146,306. The deduced product exhibited around 40% identity in amino acids with Na(+)/K(+)-ATPase alpha-subunits. A hydrophilic sequence of 285 amino acid residues that showed no homology with any sequence listed in databases existed in the M7--M8 junction of HANA. This is the first report on the primary structure of putative Na(+)-transporting ATPase from plant cells. |
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