| 人脐带血来源造血干细胞体外扩增的研究进展 |
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| 引用本文： | 董忱,赵龙,张斌,陈虎.人脐带血来源造血干细胞体外扩增的研究进展[J].中华细胞与干细胞杂志(电子版),2019,9(1):44-49. |
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| 作者姓名： | 董忱 赵龙 张斌 陈虎 |
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| 作者单位： | 1. 100071 北京，军事科学院军事医学研究院
2. 100071 北京，解放军总医院第五医学中心（原解放军第307医院）造血干细胞移植科
3. 100071 北京，全军造血干细胞研究所 造血干细胞治疗及转化研究北京市重点实验室 |
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| 基金项目： | 北京市科学技术委员会干细胞与再生医学研究项目(Z181100001818004) |
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| 摘 要： | 造血干细胞（HSCs）是血液系统中的一类成体干细胞群，具有自我更新和多谱系分化两个基本特征。造血干细胞移植（HSCT）可以治疗退行性疾病和多种血液系统疾病。脐带血来源造血干细胞（CB HSCs）是降低HLA配型要求的突破点，但单份脐带血中HSCs数量不能满足使用要求，为了获得足够数量的CB HSCs，体外扩增是一种可行的方法。近几年，学者们探索了多种体外扩增方法，包括优化细胞生长因子混合物、与基质细胞共培养及加入小分子化合物（SMCs）激动剂等。目前应用细胞因子联合小分子的扩增方法在多个临床试验中获得成功。本文对目前体外扩增CB HSCs的研究进展做一综述。
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| 关 键 词： | 脐带血 造血干细胞 体外扩增 小分子化合物 细胞因子 |
| 收稿时间： | 2018-04-11 |
Advance of ex vivo expanded human hematopoietic stem cells derived from umbilical cord blood |
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| Authors: | Chen Dong Long Zhao Bin Zhang Hu Chen |
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| Institution: | 1. Academy of Military Medical Sciences, Academy of Military Science, Beijing 100071, China
2. Department of Hematopoietic Stem Cell Transplantation, the Fifth Medical Centre of the People's Liberation Army General Hospital(Former 307th Hospital of the PLA), Beijing 100071, China
3. the Research Institute of Hematopoietic Stem Cell of the People's Liberation Army, Beijing Key Laboratory of Stem Cell Therapy and Transformation Research, Beijing 100071, China |
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| Abstract: | Hematopoietic stem cells (HSCs) are a group of adult stem cells in the blood system, which have two basic characteristics of self-renewal and multi-lineage differentiation. Hematopoietic stem cell transplantation (HSCT) can treat degenerative diseases and various blood system diseases. Umbilical cord blood (CB) HSCs do no require full HLA matching in HSCT, but the number of HSCs in a single cord blood unit cannot meet the requirements for adult recipients. In order to obtain a sufficient number of CB HSCs, ex vivo expansion is a feasible method. In recent years, reserachers have explored a variety of ex vivo expansion methods, including optimization of cytokine cocktails, co-culture with stromal cells, and addition of small molecule compounds (SMCs). At present, cytokines combined with SMCs have been successful in many clinical trials. This article reviews the advance of ex vivo expansion of HSCs derived from cord blood. |
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| Keywords: | Umbilical cord blood Hematopoietic stem cell Ex vivo expansion Small-molecule compound Cytokines |
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