多能性胚胎干细胞与DNA甲基化的关系

胚胎干细胞是一种能够维持自我更新、具有无限扩增能力的多能性干细胞。灵长类多能干细胞（iPSCs）根据其发育能力、细胞形态、基因表达谱以及表观遗传学的差异分为初始态多能干细胞（pPSCs）和原始态多能干细胞（nPSCs）。nPSCs因其容易进行基因工程处理以及体内外再生出功能组织器官等优势而在临床潜在应用上备受关注，因而有效维持ESCs的原始状态对其用于基础及临床研究具有重要意义。nPSCs的线粒体活性和自我更新能力高于pPSCs，且这两种多能性干细胞在DNA甲基化等方面都存在明显差别，DNA甲基化在nPSCs的转化及代谢中起到重要的作用。本文综述了DNA甲基化对ESCs的作用，特别是维持原始态的作用。

Correlation of pluripotency of embryonic stem cells with DNA methylation

Embryonic Stem cells is a kind of pluripotent stem cells which can maintain self-renewal and have unlimited expansion ability. Primate pluripotent stem cells (PSCs) are defined as naive- and primed- state according to their varieties in cellular, molecular, epigenetic and functional states. nPSCs had attracted much attention in clinical application because of its easy for genetic engineering and regeneration of functional tissues and organs in vitro or in vivo. Thus maintaining their na?ve state effectively is of great significance to basic and clinical research of stem cells. Numerous studies shows that na?ve PSCs had a higher mitochondrial activity and self-?renewal ability than Primed ESCs, and there were significant differences between these two kinds of pluripotent stem cellsin DNA methylation. This article provides the effect of DNA methylation on embryonic stem cells, particularly focusing on the maintenance of embryonic stem cell naive state.