聚合酶链反应快速检测胃组织和唾液中的幽门螺杆菌

针对幽门螺杆菌（ＨＰ）尿素酶Ａ基因设计一对引物进行聚合酶链反应，检测１株ＨＰ标准株和７株临床分离株均阳性，而４株其它肠道菌均阴性，特异性１００％。１０倍系列稀释试验表明敏感性达到１００ｐｇＤＮＡ水平。从３５例胃镜检查者取幽门旁组织块进行快速和常规尿素酶试验，细菌培养及ＰＣＲ检测，１５例ＨＰ阳性者ＰＣＲ检测也为阳性，其中７例阳性者有３例唾液ＰＣＲ检测为阳性，表明ＨＰ确存在于口腔中。本研究采用直接热裂解法处理临床标本，取其粗提物行ＰＣＲ，免除复杂的酚一氟仿抽提步骤，该法简便快速，且损失小，成功率高，在临床实验诊断中有推广价值。

RAPID DETECTION OF HELICOBACTER PYLORI IN GASTRIC BIOPSY AND SALIVA SAMPLES BY POLYMERASE CHAIN REACTION

By using primers based on the sequence of urease A gene of HP,a protocol was estabished to detect this bacterium in gastric biopsy and saliva samples by PCR. This assay could detect all 8 HP strains, but falled to detect other 4 enteric strains,showing it to be 100%specific.Tenfold serial dilution experiments revealed the detection of as little as 100pg DNA by this assay.HP was detected by PCR in 15 gastric biopsies from patients positive for it,and only 3 of 7 patients who tested positive by PCR of gastric biopsy were positive when a saliva sample was analyzed,In this assay,crude extraction by single-step heat lysis of clinical sampies was developed,instead of multi-step phenol chloroform extraction.The results showed it to be simple,rapid and successful, thus its value as a laboratory diagnostic test was comfirmed.