Cloning and Production of a Novel Bacteriocin, Lactococcin K, from Lactococcus lactis Subsp. lactis MY23 |
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| Authors: | Yun Seog Kim Min Ju Kim Pil Kim Jung Hoe Kim |
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| Affiliation: | (1) Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Guseong-dong, 373-1, Yusong-gu, Daejeon, 305-701, Korea;(2) LG Household & Healthcare Research R&D Institute, Jang-dong, Yuseong-gu, Daejeon, 305-343, Korea;(3) Biotechnology Major, The Catholic University of Korea, Bucheon, 420-743, Korea |
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| Abstract: | A gene encoding the antimicrobial peptide, lactococcin K, was isolated from Lactococcus lactis subsp. lactis MY23 then cloned and expressed in Escherichia coli. Because the expressed lactococcin K was formed as an inclusion body in recombinant E. coli, a fusion protein containing lactococcin K and maltose-binding protein (MBP) was produced in a soluble form. For high-level production of lactococcin K, we performed a pH-stat fed-batch culture to produce 43,000 AU lactococcin K ml−1 in 12 h. Revisions requested 3 November 2005; Revisions received 7 December 2005 |
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| Keywords: | cloning Kimchi lactococcin K Lactococcus lactis subsp. lactis |
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