Inteins and affinity resin substitutes for protein purification and scale up |
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Authors: | Mahmoud?Reza?Banki Email author" target="_blank">David?W?WoodEmail author |
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Institution: | (1) Department of Chemical Engineering, Princeton University, A213 E-QUAD, 08544 Princeton, NJ, USA |
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Abstract: | The development of self-cleaving fusion-tag technology has greatly simplified the purification of recombinant proteins at
laboratory scale. The self-cleaving capability of these tags has recently been combined with additional purification tags
to generate novel and convenient protein purification methods at a variety of scales. In this review, we describe some of
these methods, and provide a rudimentary economic analysis of hypothetical large-scale applications. This work is expected
to provide a rough outline for the evaluation of these methods for large-scale bioprocessing of a variety of products. |
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