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Hydrolytic cleavage of double-strand DNA by the water-soluble dicobalt(III) complexes of 1,4,7-Triazacyclononane-N-acetate |
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| Authors: | Jing Qian Xiaofang Ma Wen Gu Xin Liu |
| Affiliation: | a Department of Chemistry, Nankai University, Tianjin 300071, PR China b College of Chemistry and Life Science, Tianjin Normal University, Tianjin, 300387, PR China c College of Basic Medicine Science, Zhengzhou University, Zhengzhou 450001, PR China |
| Abstract: | Three water-soluble dicobalt(III) complexes, [Co2L2(µ-OH)2](ClO4)2·5H2O (1), [Co2L2(µ-OH)2](ClO4)2·CH3OH·H2O(2); [Co2L2(µ-OH)2](ClO4)2·4H2O(3) (L = 1,4,7-triazacyclononane-N-acetate monoanion), were prepared to serve as nuclease mimics. The complexes were characterized by X-ray, IR and UV-vis spectroscopy as well as ESI-MS. Three complexes exhibit similar structures, just with different solvent molecules. The electrospray mass spectrum of 1 in solution indicates that dinuclear ion [Co2L2(µ-OH)2-H+] + (4) is the active species. In the absence of any reducing agent, the complexes cleave plasmid pBR322 DNA was performed and its hydrolytic mechanism was demonstrated with radical scavengers, anaerobic reaction and T4 ligase. The kinetic aspects of DNA cleavage under pseudo- or true-Michaelis-Menten conditions are also detailed, kinetic parameters (kcat, KM) were calculated to be 3.57 h− 1, 6.92 × 10− 4 M; 0.28 h− 1, 1.9 × 10− 5 M for 4, respectively. |
| Keywords: | Cobalt(III) complexes 1,4,7-triazacyclononane-N-acetate μ-Hydroxo Hydrolytic cleavage |
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