Synthesis, characterization and antibacterial activity of Fe, Co, Cu and Zn complexes probed by transmission electron microscopy |
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Authors: | Christiane Fernandes,Adolfo Horn Jr.,Vagner M. de Assis,Luciana da S. Mathias,Adailton J. Bortoluzzi,Joã o Carlos Aquino Almeida |
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Affiliation: | a Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense, 28013-602, Campos dos Goytacazes, RJ, Brazilb Laboratório de Sanidade Animal, Universidade Estadual do Norte Fluminense, 28013-602, Campos dos Goytacazes, RJ, Brazilc Departamento de Química, Universidade Federal de Santa Catarina, 88040-900, Florianópolis, SC, Brazild Laboratório de Biologia Estrutural, Instituto Oswaldo Cruz, Fiocruz, 21040-360, Rio de Janeiro, RJ, Brazile Laboratório de Fisiologia e Bioquímica de Microrganismos, Universidade Estadual do Norte Fluminense, 28013-602, Campos dos Goytacazes, RJ, Brazilf Chemistry Department and Laboratory for Biological Mass Spectrometry, Texas A&M University, 77843-3255, College Station, TX, United States |
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Abstract: | We synthesized iron(III), cobalt(II), copper(II) and zinc(II) complexes [FeIII(HBPClNOL)Cl2]·H2O (1), [CoII(H2BPClNOL)Cl2] (2), [CuII(H2BPClNOL)Cl]Cl·H2O (3), and [ZnII(HBPClNOL)Cl] (4), where H2BPClNOL is the ligand (N-(2-hydroxybenzyl)-N-(2-pyridylmethyl)[(3-chloro)(2-hydroxy)]propylamine). The complexes obtained were characterized by elemental analysis, IR and UV-visible spectroscopies, electrospray ionization mass spectrometry (ESI-MS), tandem mass spectrometry (MS/MS), and cyclic voltammetry. X-ray diffraction studies were performed for complexes (3) and (4) revealing the presence of mononuclear and dinuclear structures in solid state for (3). However, the zinc complex is mononuclear in solid state. Biological studies of complexes (1)-(4) were carried out in vitro for antimicrobial activity against nine Gram-positive bacteria (Staphylococcus aureus strains RN 6390B, COL, ATCC 25923, Smith Diffuse, Wood 46, enterotoxigenic S. aureus FRI-100 (SEA+), FRI S-6 (SEB+) and SEC FRI-361) and animal strain S. aureus LSA 88 (SEC/SED/TSST-1+). The following sequence of inhibition promoted by the complexes was observed: (4) > (2) > (3) > (1), showing the effect of the metal on the biological activity. To directly observe the morphological changes of the internal structure of bacterial cells after the treatment, transmission electron microscopy (TEM) was employed. For the most active complex [ZnII(HBPClNOL)Cl] (4), granulation deposits around the genetic material and internal material leaking were clearly detected. |
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Keywords: | FeIII, CoII, CuII, ZnII complexes Antibacterial activity S. aureus ESI-MS/MS X-ray Transmission electron microscopy |
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