Common basis for the mechanism of metallo and non-metallo KDO8P synthases |
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Authors: | Peng Tao H. Bernhard Schlegel Domenico L. Gatti |
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Affiliation: | a Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, United Statesb Department of Chemistry, Wayne State University, 5101 Cass Ave Detroit, Michigan 48202, United States |
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Abstract: | The three-dimensional structures of metal and non-metal enzymes that catalyze the same reaction are often quite different, a clear indication of convergent evolution. However, there are interesting cases in which the same scaffold supports both a metal and a non-metal catalyzed reaction. One of these is 3-deoxy-D-manno-octulosonate 8-phosphate (KDO8P) synthase (KDO8PS), a bacterial enzyme that catalyzes the synthesis of KDO8P and inorganic phosphate (Pi) from phosphoenolpyruvate (PEP), arabinose 5-phosphate (A5P), and water. This reaction is one of the key steps in the biosynthesis of bacterial endotoxins. The evolutionary tree of KDO8PS is evenly divided between metal and non-metal forms, both having essentially identical structures. Mutagenesis and crystallographic studies suggest that one or two residues at most determine whether or not KDO8PS requires a metal for function, a clear example of “minimalist evolution”. Quantum mechanical/molecular mechanical (QM/MM) simulations of both the enzymatic and non-enzymatic synthesis of KDO8P have revealed the mechanism underlying the switch between metal and non-metal dependent catalysis. The principle emerging from these studies is that this conversion is possible in KDO8PS because the metal is not involved in an activation process, but primarily contributes to orienting properly the reactants to lower the activation energy, an action easily mimicked by amino acid side-chains. |
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Keywords: | KDO8PS Metalloenzymes Mechanism QM/MM Potential energy surface Evolution |
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