Effect of rioprostil and colchicine on CC14-acute liver damage in rats. Relationship with plasma membrane lipids |
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Authors: | Marisabel Mourelle, Jos Luis Amezcua,Enrique Hong |
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Affiliation: | Marisabel Mourelle, JoséLuis Amezcua,Enrique Hong |
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Abstract: | The effects of colchicine (10 g/day p.o. for 7 days) and rioprostil (2-decarboxy, 2-hydroxymethyl-15-deoxy-16-RS-hydroxy-16-methyl-prostaglandin-E1) (20 g/kg s.c., a single dose) on the enzymatic and histological markers of acute liver damage were studied in rats intoxicated with a single oral dose of CC14. The rats were sacrificed 24 h after CC14. The lipid composition of the liver plasma membranes was also determined. The increase in Alk. Phosp., GGTP and GPT activities and bilirubin concentration in serum as well as the histological images produced by CC14 were equally prevented by the treatments with colchicine or rioprostil. CC14 changed the lipid composition of the liver plasma membrane by increasing PI and PC and decreasing SM, PS and PEA. There was a decrease in the cholesterol/phospholipid ratio at the expense of a reduction of cholesterol/protein ratio and elevation in phospholipid/protein ratio. Colchicine and rioprostil also prevented these lipid alterations. The results suggest that the plasma membrane is an important site of action of CC14 and of the 2 drugs studied. We postulate that the plasma membrane rather than other organelles is the target for the cytoprotective actions of prostaglandins. |
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