Cell cycle analysis and synchronization of the TN-368 insect cell line |
| |
Authors: | D. E. Lynn W. F. Hink |
| |
Affiliation: | (1) Department of Entomology, The Ohio State University, 43210 Columbus, Ohio |
| |
Abstract: | Summary A cell cycle analysis of theTrichoplusia ni (TN-368) insect cell line is described. By means of autoradiography and percent labeled metaphase data, the cell cycle parameters were determined to be as follows: S, 4.5 hr; G2, 8.5 hr; M, 0.5 hr; G1, 1.0 hr; the total cell time being 14.5 hr. A synchronization procedure using 50mm thymidine in a double block procedure was used to provide a method of obtaining a large number of cells in particular cell cycle phases, especially S and G2. This work was supported in part by U.S. Environmental Protection Agency Grant R-802516. |
| |
Keywords: | insect cells cell cycle synchronization |
本文献已被 SpringerLink 等数据库收录! |
|