5-hydroxylation of benzimidazole by micromycetes II. Optimisation of production with Absidia spinosa

Summary The bioconversion of benzimidazole to 5-hydroxybenzimidazole was investigated as a function of the physiological state of the fungus and culture conditions. Using an inoculum composed only of spores, the addition of benzimidazole at different times of development led to a better definition of optimal physiological conditions for obtaining a good hydroxylation yield. The relationship between the degree of hydroxylation and that of culture medium aeration, was shown by the use of four different conditions: flowing medium on an inert support, in static, agitated or highly aerated medium.