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| 太湖日本沼虾野生群体遗传结构的微卫星分析 | |
| 引用本文： | 冯建彬,吴春林,马克异,丁怀宇,华雪铭,李家乐. 太湖日本沼虾野生群体遗传结构的微卫星分析[J]. 应用生态学报, 2011, 22(6): 1606-1614 |
| 作者姓名： | 冯建彬 吴春林 马克异 丁怀宇 华雪铭 李家乐 |
| 作者单位： | 1. 上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海,201306 2. 江苏省环洪泽湖生态农业生物技术重点实验室,江苏淮安,223300 3. 上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306;上海高校水产养殖学E-研究院,上海201306 |
| 基金项目： | 国家自然科学基金项目(31001111); 上海高校选拔培养优秀青年教师科研专项基金项目(SSC08004); 上海市科委重点科技攻关计划项目(073205111); 江苏省苏北科技发展计划项目(BN2009036)资助 |
| 摘 要： | 利用8个高度多态性的微卫星位点分析了太湖日本沼虾野生群体的遗传结构.结果表明:在15个群体中至少有3个位点经Bonferroni校正后显示杂合不足,显著偏离了HardyWeinberg平衡;15个群体中观测杂合度均大于0.683,显示出较高的遗传多样性水平,但其波动明显,如太湖东、南部的渡口和陆巷等群体的遗传多样性高于西、北部的华庄和洋渚等群体;突变-漂移平衡分析结果显示,15个群体中部分位点杂合显著过剩,偏离了突变-漂移平衡,且近期曾经历过瓶颈效应,群体数量曾经下降;群体间AMOVA分析表明,太湖日本沼虾群体间遗传分化程度较低(FST=0.011),98.9%的遗传变异来自群体内,1.1%来自群体间,并没有形成显著的遗传结构,在种质资源保护和管理上可视作一个单元;华庄与吴塘门群体间DA遗传距离达到0.206,已接近种间分类界限,故太湖日本沼虾种质资源可持续利用工作仍须深入的研究. |
| 关 键 词： | 日本沼虾 太湖 微卫星 遗传结构 |
Genetic structure of wild Macrobrachium nipponense populations in Taihu Lake based on microsatellite analysis |
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| Feng Jian-Bin,Wu Chun-Lin,Ma Ke-Yi,Ding Huai-Yu,Hua Xue-Ming,Li Jia-Le. Genetic structure of wild Macrobrachium nipponense populations in Taihu Lake based on microsatellite analysis[J]. The journal of applied ecology, 2011, 22(6): 1606-1614 | |
| Authors: | Feng Jian-Bin Wu Chun-Lin Ma Ke-Yi Ding Huai-Yu Hua Xue-Ming Li Jia-Le |
| Affiliation: | FENG Jian-bin1,WU Chun-lin1,MA Ke-yi1,DING Huai-yu2,HUA Xue-ming1,LI Jia-le1,3(1Ministry of Education Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Shanghai 201306,China,2Jiangsu Province Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake,Huai'an 223300,Jiangsu,3Aquaculture Division,E-Institute of Shanghai Universities,China) |
| Abstract: | By using eight highly polymorphic microsatellite DNA loci,this paper analyzed the genetic structure of wild Macrobrachium nipponense populations in Taihu Lake.For the 15 M.nipponense populations in the Lake,there were at least three of the loci presenting heterozygosity deficiency and obvious deviation from Hardy-Weinberg equilibrium after Bonferroni correction.The observed heterozygosity values of the 15 populations were all above 0.683,displaying a high genetic diversity,but the diversity varied obviously... |
| Keywords: | Macrobrachium nipponense Taihu Lake microsatellite genetic structure |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |