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| 缺硫对镉胁迫下水稻幼苗非蛋白巯基物质含量和谷胱甘肽硫转移酶活性的影响 | |
| 作者姓名： | Gao KH Ge Y Zhang CH |
| 作者单位： | 1. 南京农业大学生命科学学院,南京,210095 2. 南京农业大学资源与环境科学学院,南京,210095 3. 南京农业大学生命科学实验中心元素与生命科学研究合作示范实验室,南京,210095 |
| 基金项目： | 国家自然科学基金项目(30700479); 教育部高等学校博士点科研基金项目(20090097110035)资助 |
| 摘 要： | 通过设置缺硫(S)处理,研究了镉(Cd)胁迫下水稻生长情况、幼苗Cd和非蛋白巯基含量以及谷胱甘肽硫转移酶(GST)活性的动态变化.结果表明:Cd胁迫明显抑制了水稻生长,显著诱导了巯基物质非蛋白巯基(NPT)、谷胱甘肽(GSH)、植物螯合肽(PC)]的合成,GST活性表现出先升后降的趋势.缺S处理下,尽管水稻根部对Cd的吸收和向地上部的转运都有所增加,但Cd胁迫程度并未明显增强,巯基物质含量明显降低,根部GST活性提高.表明巯基物质和GST在水稻抗Cd胁迫过程中互为补充,在一定程度上减轻了Cd的毒性效应. |
| 关 键 词： | 缺硫 镉 水稻 非蛋白巯基 谷胱甘肽硫转移酶 |
Effects of sulfur starvation on the non-protein thiol content and glutathione S-transferase activity of rice seedlings under cadmium stress |
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| Gao KH,Ge Y,Zhang CH.Effects of sulfur starvation on the non-protein thiol content and glutathione S-transferase activity of rice seedlings under cadmium stress[J].Chinese Journal of Applied Ecology,2011,22(7):1796-1802. | |
| Authors: | Gao Ke-hui Ge Ying Zhang Chun-hua |
| Institution: | GAO Ke-hui1,GE Ying2,ZHANG Chun-hua3(1College of Life Science,Nanjing Agricultural University,Nanjing 210095,China,2College of Resources and Environmental Sciences,3Demonstration Laboratory of Elements and Life Science Research,Laboratory Centre of Life Science,China) |
| Abstract: | By installing a treatment of sulfur starvation,this paper studied the dynamic changes of rice seedling growth,cadmium(Cd) and non-protein thiol(NPT) contents,and glutathione S-transferase(GST) activity under Cd stress.Cd stress inhibited the seedling growth obviously,induced the synthesis of sulfhydryl(-SH) compounds,including non-protein thiol,glutathione,and phytochelatins,and made the GST activity decreased after an initial increase.Sulfur starvation somewhat increased the Cd uptake and translocation,but... |
| Keywords: | sulfur starvation cadmium rice non-protein thiol glutathione Stransferase |
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