Expression of 15-lipoxygenase-1 in human nasal epithelium: its implication in mucociliary differentiation |
| |
Authors: | Kim Kyung-Su Chun Hee-Sun Yoon Joo-Heon Lee Jeung Gweon Lee Joo-Hwan Yoo Jong-Bum |
| |
Institution: | Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea. ydrhinol@yumc.yonsei.ac.kr |
| |
Abstract: | 15-lipoxygenase-1 (15-LO-1) is involved in the differentiation of human tracheobronchial epithelial cells. Here, we investigated the relation between 15-LO-1 expression and the differentiation of human nasal epithelium. In retinoic acid (RA)-sufficient culture media, 15-LO-1 expression in normal human nasal epithelial cell time-dependently increased, but its expression was undetectable in RA-deficient culture media. Moreover, in RA-deficient culture media, IL-4 at 1 ng/ml concentration time-dependently induced 15-LO-1 expression. In addition, MUC8 gene expression, a marker of mucociliary differentiation, was up-regulated by 15-LO-1, which was itself induced by IL-4. In murine nasal mucosa, the expression of leukocyte type-12-LO, a functional equivalent of 15-LO-1, reduced after postnatal day 7. Our findings suggest that 15-LO-1 is related to the differentiation of human nasal epithelium, and that it may mediate the mucociliary differentiation of human nasal epithelium. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|