Evolution of digestibility by Hind III: an analysis by light and electron microscopy |
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| Authors: | J Méndez A M González A M Insua V J Goyanes |
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| Institution: | (1) Departamento de Genética, Colegio Universitario de La Coruña, Universidad de Santiago, 15071 La Coruña, Spain;(2) Sección de Genética, Hospital  T. Herrera (INSALUD), 15006 La Coruña, Spain |
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| Abstract: | Digestion of Chinese hamster metaphase chromosomes from the Don cell line by Hind III restriction endonuclease followed by Giemsa staining were analysed by light and electron microscopy. The evolution of digestibility was studied and four digestion stages were characterized by different levels of chromosome structure. Three different condensation stages were established according to morphological criteria of length, width and separation among chromatids. It was observed that there are statistically significant differences in the digestion progress at the three condensation stages previously defined. |
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