Aginoside saponin,a potent antifungal compound,and secondary metabolite analyses from Allium nigrum L. |
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| Authors: | Abdelrahman Mostafa Jogaiah Sudisha Magdi El-Sayed Shin-ichi Ito Tsuyoshi Ikeda Naoki Yamauchi Masayoshi Shigyo |
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| Institution: | 1. Laboratory of Vegetable Crop Science, Department of Biological and Environmental Sciences, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan;2. The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-Minam, Tottori 680-8553, Japan;3. Laboratory of Molecular Plant Pathology, Department of Biological and Environmental Sciences, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan;4. Unit of Environmental Studies and Development, Faculty of Science, Aswan University, Aswan 81528, Egypt;5. Downy Mildew Research Laboratory, Department of Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India;6. Department of Natural Medicine, Faculty of Pharmaceutical Sciences, Sojo University, Kumamoto, Japan |
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| Abstract: | The HPLC and spectral analyses of cysteine sulfoxides (CSOs), total polyphenols (TP), and total saponins revealed quantitative variations within the different organs of Allium nigrum L. A large accumulation of CSOs was detected in the bulb (0.367 mg/g fw), of TP in the leaf (116.05 mg CE/100 g fw), and of saponins in the root (19.38 mg/g dw). Phytochemical and chromatographical investigations of A. nigrum root extract led to the isolation of a spirostane-type glycoside or aginoside. The structure was elucidated by spectroscopic analysis (2D NMR, FABMS, HR-ESI-MS). The structure of the aginoside was identified as 25(R,S)-5α-spirostan-2α,3β,6β-trio1-3-O-β-d-glucopyranosyl-(1→2)-O-β-d-xylopyranosyl-(1→3)]-O-β-d-glucopyranosyl-(1→4)-β-d-galactopyranoside. The highest content of aginoside, 2.9 mg/g dw, was detected in the root. The in vitro and in vivo antifungal activity of aginoside was evaluated for the first time against phytopathogens. This compound showed significant (P < 0.05) antifungal activity depending on the concentration. |
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| Keywords: | Saponin Aginoside Antifungal activity Cysteine sulfoxides Polyphenols |
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