Identification of differential expressed proteins and characterization their mRNA expression in thermally stressed Apostichopus japonicus |
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| Authors: | Peng Zhang Yali Lu Chenghua Li Xiurong Su Zhonghua Wang Chunhua Jin Ye Li Taiwu Li |
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| Institution: | 1. School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China;2. Ningbo City College of Vocational Technology, Ningbo, 315100, PR China;1. School of Marine Sciences, Ningbo University, Ningbo 315211, China;2. Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China;1. CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;3. The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Qingdao 266003, China;4. Wyoming Seminary College Preparatory School, Kingston, PA 18704, USA;5. China Rural Technology Development Center, Beijing 100045, China |
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| Abstract: | In this study, we present a comparative proteomic analysis of the global protein expression changes in sea cucumber after 7 days exposure at 25 °C. Using two-dimensional electrophoresis followed by MALDI-TOF MS/MS, 27 protein spots with significant differences in abundance were identified and characterized. The identified proteins belonged primarily to the following four functional categories: cytoskeletal, material and energy metabolism, calcium homeostasis and extracellular matrix. The mRNA expression levels of 7 differentially expressed proteins were further assessed by qRT-PCR. The expression levels of 6 genes, including collagen, ATP synthase, major yolk protein, ferritin, nectin and protein disulfide isomerase showed significant differences under thermal stress, and among them, only two genes—ATP synthase and major yolk protein—showed consistent levels of protein and mRNA expression. Our results offer insight into the complex changes in protein turnover during higher temperature exposure in sea cucumber. |
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