In vitro growth inhibitory effects of 13,28-epoxyoleanane triterpene saponins in cancer cells |
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Authors: | Mohamed Haddad Anne-Cécile Lelamer Laetitia Moreno Y Banuls Pedro Vasquez Maelle Carraz Abraham Vaisberg Denis Castillo Michel Sauvain Rosario Rojas Robert Kiss |
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Institution: | 1. Université de Toulouse, Université Paul Sabatier, Pharma-Dev UMR 152, Faculté de Pharmacie, 35 Chemin des maraîchers, F-31062, Toulouse cedex 9, France;2. Institut de Recherche pour le Développement (IRD), Pharma-Dev UMR 152, Mission IRD, Casilla 18-1209, Lima, Peru;3. Laboratoire de Toxicologie, Faculté de Pharmacie, Université Libre de Bruxelles (ULB), Brussels, Belgium;4. Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru |
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Abstract: | Two new 13,28-epoxyoleanane triterpene saponins, magnosides A (1) and B (2), were isolated from the 95% ethanolic extract of Cybianthus magnus (Mez) Pipoly roots. Their structures were deduced by a combination of spectral analyses and chemical evidences as compared to data reported in the literature. The hemolytic activity of both compounds was measured. Compound 1 was shown to exhibit the strongest hemolytic activity with a HD50 of 3.8 μM followed by 2 with a HD50 of 33.5 μM. The bioactivity of compounds 1 and 2 was also evaluated in vitro against different cellular models including Mycobacterium tuberculosis, Leishmania amazonensis axenic amastigotes, mouse peritoneal macrophages and eight cancer cell lines. While neither of the tested compounds displayed any activity against M. tuberculosis, both exhibited anti-leishmanial activity against axenic amastigotes as well as in vitro growth inhibitory activity against all tested cancer cell lines with IC50 growth inhibitory concentrations ranging between 4 μM and 33 μM. The compounds displayed similar growth inhibitory activity in cancer cell lines sensitive to pro-apoptotic stimuli versus those displaying various levels of resistance to such stimuli. Quantitative videomicroscopy analyses revealed that compounds 1 and 2 are cytotoxic. |
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