Specificity of anti-nucleoside antibodies (Short Communication) |
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Authors: | Mir Z. Humayun and T. Mathai Jacob |
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Affiliation: | Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India |
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Abstract: | The method of conjugation of a nucleoside or related compound to a carrier protein may have a significant effect on the specificity of the antibodies elicited. It is demonstrated, by means of the membrane-filtration assay, that anti-isopentenyladenosine antibodies produced by the ;periodate procedure' are much more reactive with the periodate-oxidized form of the nucleoside than with the parent compound. In addition, the simplicity and specificity of the assay used suggests its use as a sensitive radioimmunoassay for this multifunctional nucleoside. |
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